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complete egm-2 endothelial cell growth medium-2  (Lonza)


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    Lonza complete egm-2 endothelial cell growth medium-2
    Complete Egm 2 Endothelial Cell Growth Medium 2, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+endothelial+cell+growth+medium-2+egm-2/egm+2+medium/pmc12030166-86-8-17
    Average 90 stars, based on 1 article reviews
    complete egm-2 endothelial cell growth medium-2 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: METRNL exerts cytoprotective effects on EPCs via regulation of the E2F1-TXNIP axis in obese limb ischemia.
    Article Snippet: Background: Obesity increases cardiovascular disease risk by impairing angiogenesis, primarily through dysfunction of endothelial progenitor cells (EPCs).. METRNL, a recently identified secreted protein, exhibits diverse biological activities.. However, its impact on EPC function and its role in obesity-related microvascular dysfunction remain unclear.

    Article Title: Human Myocardial Pericytes: Multipotent Mesodermal Precursors Exhibiting Cardiac Specificity
    Article Snippet: Cell culture and cell labelling Freshly sorted hHPs were initially seeded at 2×10 4 cells/cm 2 in complete endothelial cell growth medium-2 (EGM-2, Lonza) and cultured at 37°C for 2 weeks on plates coated with 0.2% gelatin (Calbiochem) until confluence. hHPs were then detached by 0.25% trypsin-EDTA (Invitrogen, Life Technologies) treatment for 10 min at 37°C, split at a 1:3 ratio, and seeded on uncoated plates with pericyte maintenance medium: DMEM high glucose (Invitrogen, Life Technologies) supplemented with 20% FBS and 1% P/S.

    Article Title: Human Myocardial Pericytes: Multipotent Mesodermal Precursors Exhibiting Cardiac Specificity
    Article Snippet: Freshly sorted hHPs were initially seeded at 2×10 4 cells/cm 2 in complete endothelial cell growth medium-2 (EGM-2, Lonza) and cultured at 37°C for 2 weeks on plates coated with 0.2% gelatin (Calbiochem) until confluence. hHPs were then detached by 0.25% trypsin-EDTA (Invitrogen, Life Technologies) treatment for 10 min at 37°C, split at a 1:3 ratio, and seeded on uncoated plates with pericyte maintenance medium: DMEM high glucose (Invitrogen, Life Technologies) supplemented with 20% FBS and 1% P/S.



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    Untargeted differential proteomics analysis to investigate the impact of enhanced local cell density after sound patterning of centimeter-scale microcapillary networks. (a) At day 0, Green Fluorescent Protein-Expressing human umbilical vein endothelial cells (GFP-HUVEC) and human pericytes from placenta (hPC-PL) were collected and mixed at a ratio of 10:1 in a single-cell suspension made in fibrin, then dispensed into the sound patterning chamber with a round geometry. Before gelation, Faraday waves were generated at the liquid-air interface upon application of 54 Hz frequency vibration, and sound-induced hydrodynamic forces condensed the cells into reproducible pattern of 4 concentric circles in less than 2 min. At day 5, the concentric circles pattern samples were washed with PBS and incubated at 37 °C with 1 ml of a 2 mg mL −1 Nattokinase solution prepared in 1 mM EDTA PBS. Cellular material was collected in pellets, which underwent protein digestion. The digested peptides were then analyzed by mass spectrometry. (b) Proteomic analysis of the patterned capillaries after 5 days in culture revealed over 900 differentially expressed proteins compared to the stationary condition (random). 643 proteins were up-regulated and 261 down-regulated in the patterned microcapillaries compared to the random condition ( n = 3). The heatmap (c) shows the 50 most significant proteins, and (d) the Principal Components Analysis (PCA) of the dataset reduced to two dimensions shows spatial separation between the random and patterned conditions.

    Journal: Biomaterials and Biosystems

    Article Title: Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement

    doi: 10.1016/j.bbiosy.2024.100094

    Figure Lengend Snippet: Untargeted differential proteomics analysis to investigate the impact of enhanced local cell density after sound patterning of centimeter-scale microcapillary networks. (a) At day 0, Green Fluorescent Protein-Expressing human umbilical vein endothelial cells (GFP-HUVEC) and human pericytes from placenta (hPC-PL) were collected and mixed at a ratio of 10:1 in a single-cell suspension made in fibrin, then dispensed into the sound patterning chamber with a round geometry. Before gelation, Faraday waves were generated at the liquid-air interface upon application of 54 Hz frequency vibration, and sound-induced hydrodynamic forces condensed the cells into reproducible pattern of 4 concentric circles in less than 2 min. At day 5, the concentric circles pattern samples were washed with PBS and incubated at 37 °C with 1 ml of a 2 mg mL −1 Nattokinase solution prepared in 1 mM EDTA PBS. Cellular material was collected in pellets, which underwent protein digestion. The digested peptides were then analyzed by mass spectrometry. (b) Proteomic analysis of the patterned capillaries after 5 days in culture revealed over 900 differentially expressed proteins compared to the stationary condition (random). 643 proteins were up-regulated and 261 down-regulated in the patterned microcapillaries compared to the random condition ( n = 3). The heatmap (c) shows the 50 most significant proteins, and (d) the Principal Components Analysis (PCA) of the dataset reduced to two dimensions shows spatial separation between the random and patterned conditions.

    Article Snippet: Green Fluorescent Protein-expressing Human Umbilical Vein Endothelial Cells (GFP-HUVEC, cAP-0001GFP) were purchased from Angio-Proteomie, Boston, USA, and expanded in Complete Endothelial Cell Growth Medium (EGM-2, Lonza Group AP, Switzerland) in pre-coated culture flasks (Quick Coating Solution, Angio-Proteomie).

    Techniques: Expressing, Suspension, Generated, Incubation, Mass Spectrometry